解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::Switchgrass (Panicum virgatum L.; family Poaceae) is a warm-season C4 perennial grass. Tillering plays an important role in determining the morphology of aboveground parts and the final biomass yield of switchgrass. Auxin distribution in plants can affect a variety of important growth and developmental processes, incl...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/1678-4685-GMB-2014-0300
更新日期:2016-03-01 00:00:00
abstract::Trypoxylon is a genus of solitary crabronid wasps whose population genetics is poorly known. The purpose of the present study was to investigate the genetic variation and differentiation among five populations of Trypoxylon albitarse, a species widely distributed throughout the Neotropics, with records from Panama to ...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-475738420150097
更新日期:2015-12-01 00:00:00
abstract::Phenotypic plasticity is a hallmark of the caste systems of social insects, expressed in their life history and morphological traits. These are best studied in bees. In their co-evolution with angiosperm plants, the females of corbiculate bees have acquired a specialized structure on their hind legs for collecting pol...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-475738320140393
更新日期:2015-07-01 00:00:00
abstract::Peanut (Arachis hypogaea) is the fifth most produced oil crop worldwide. Besides lack of water, fungal diseases are the most limiting factors for the crop. Several species of Arachis are resistant to certain pests and diseases. This study aimed to successfully cross the A-genome with B-K-A genome wild species previous...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-475738320140376
更新日期:2015-07-01 00:00:00
abstract::This study presents evidence, using sequences of ribosomal 16S and COI mtDNA, for the presence of two mitochondrial genomes in Perumytilus purpuratus. This may be considered evidence of doubly uniparental mtDNA inheritance. The presence of the two types of mitochondrial genomes differentiates females from males. The F...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47573822201420140262
更新日期:2015-05-01 00:00:00
abstract::The functional role of IGFBP5 in breast cancer is complicated. Experimental and bioinformatics studies have shown that IGFBP5 is targeted by miR-140-5p and miR-193b, although this has not yet been proven in clinical samples. The aim of this study was to evaluate the expression of miR-140-5p and miR-193b in breast canc...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-475738120140167
更新日期:2015-03-01 00:00:00
abstract::Jatropha gossypiifolia L. (Euphorbiaceae), popularly known as cotton-leaf physicnut, is a milky shrub notable for its medicinal properties. The present study aimed to evaluate the toxic, cytotoxic and genotoxic effects of the latex of J. gossypiifolia, using Allium cepa L. as test system. Seeds of A. cepa were exposed...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-475738120140162
更新日期:2015-03-01 00:00:00
abstract::Indica and japonica are two main subspecies of Asian cultivated rice (Oryza sativa L.) that differ clearly in morphological and agronomic traits, in physiological and biochemical characteristics and in their genomic structure. However, the proteins and genes responsible for these differences remain poorly characterize...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572014005000015
更新日期:2014-10-01 00:00:00
abstract::In this study, we investigated the influence of two SNPs (rs846910 and rs12086634) of the HSD11B1 gene that encodes 11β-hydroxysteroid dehydrogenase type 1(11β-HSD1), the enzyme that catalyzes the conversion of cortisol to cortisone, on variables associated with obesity and metabolic syndrome in 215 individuals of bot...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/s1415-47572014000400003
更新日期:2014-09-01 00:00:00
abstract::This study evaluated the diversity of cultivable plant growth-promoting (PGP) bacteria associated with apple trees cultivated under different crop management systems and their antagonistic ability against Colletotrichum gloeosporioides. Samples of roots and rhizospheric soil from apple trees cultivated in organic and ...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/s1415-47572014000400013
更新日期:2014-09-01 00:00:00
abstract::The aim of the present study was to investigate the association between HPV-DNA and micronucleus (MN) frequency in women with normal cervical cytology. A total of 158 normal cervical smears were analyzed cytologically. The HPV genome was amplified using the GP5+/bioGP6+ consensus primers. HPV-DNA of high-risk types 16...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/s1415-47572014005000010
更新日期:2014-06-01 00:00:00
abstract::Epidermal growth factor (EGF), a multifunctional growth factor, is a regulator in a wide variety of physiological processes. EGF plays an important role in the regulation of liver regeneration. This study was aimed at investigating the methylation level of EGF gene throughout liver regeneration. DNA of liver tissue fr...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/s1415-47572014000300017
更新日期:2014-06-01 00:00:00
abstract::The Latin American School of Human and Medical Genetics (ELAG) is the main course of its kind in the genetics field in Latin America. Here we describe the main challenges regarding the organization of such event, including how we obtain funding and how we proceed with student selection. Thus, we aim to share our exper...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/s1415-47572014000200002
更新日期:2014-03-01 00:00:00
abstract::Machado-Joseph disease (MJD) is an autosomal dominant, late-onset neurological disorder and the most common form of spinocerebellar ataxia (SCA) worldwide. Diagnostic genetic testing is available to detect the disease-causing mutation by direct sizing of the CAG repeat tract in the ataxin 3 gene. Presymptomatic testin...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/s1415-47572014000200012
更新日期:2014-03-01 00:00:00
abstract::The Serrasalmidae family is composed of a number of commercially interesting species, mainly in the Amazon region where most of these fishes occur. In the present study, we investigated the genomic organization of the 18S and 5S rDNA and telomeric sequences in mitotic chromosomes of four species from the basal clade o...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/s1415-47572014000100009
更新日期:2014-03-01 00:00:00
abstract::In this work we describe the establishment of mesenchymal stem cells (MSCs) derived from embryonic stem cells (ESCs) and the role of bFGF in adipocyte differentiation. The totipotency of ESCs and MSCs was assessed by immunofluorescence staining and RT-PCR of totipotency factors. MSCs were successfully used to induce o...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/s1415-47572014000100019
更新日期:2014-03-01 00:00:00
abstract::Sickle cell anemia is a genetic disease with high prevalence in people of African descent. There are five typical haplotypes associated with this disease and the haplotypes associated with the beta-globin gene cluster have been used to establish the origin of African-descendant people in America. In this work, we dete...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572013000400005
更新日期:2013-12-01 00:00:00
abstract::The loss of soybean yield to Brazilian producers because of a water deficit in the 2011-2012 season was 12.9%. To reduce such losses, molecular biology techniques, including plant transformation, can be used to insert genes of interest into conventional soybean cultivars to produce lines that are more tolerant to drou...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572013000400015
更新日期:2013-12-01 00:00:00
abstract::The genus Phanaeus is included in the tribe Phanaeini, one of the most diverse tribes within the subfamily Scarabaeinae in terms of chromosomal characteristics. However, so far the species of this genus were not studied with differential cytogenetic techniques, limiting any inference of the probable mechanisms respons...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572013005000031
更新日期:2013-09-01 00:00:00
abstract::Ample evidence suggests that cancer is triggered by mutagenic damage and diets or supplements capable of reducing such incidences can be related to the prevention of neoplasy development or to an improvement in life quality of patients who undergo chemotherapy. This research aimed to evaluate the antimutagenic and ant...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572013005000028
更新日期:2013-09-01 00:00:00
abstract::During the last decade, microsatellites (short tandem repeats or STRs) have been successfully used for animal genetic identification, traceability and paternity, although in recent year single nucleotide polymorphisms (SNPs) have been increasingly used for this purpose. An efficient SNP identification system requires ...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572013000200008
更新日期:2013-07-01 00:00:00
abstract::Tobacco farmers are routinely exposed to complex mixtures of inorganic and organic chemicals present in tobacco leaves. In this study, we examined the genotoxicity of tobacco leaves in the snail Helix aspersa as a measure of the risk to human health. DNA damage was evaluated using the micronucleus test and the Comet a...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572013005000020
更新日期:2013-07-01 00:00:00
abstract::Cytogenetic studies in birds are still scarce compared to other vertebrates. Woodcreepers (Dendrocolaptidae) are part of a highly specialized group within the Suboscines of the New World. They are forest birds exclusive to the Neotropical region and similar to woodpeckers, at a comparable evolutionary stage. This pape...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572013000100010
更新日期:2013-03-01 00:00:00
abstract::The PPARγ nuclear receptor regulates the expression of genes involved in lipid and carbohydrate metabolism, and it has protective effects in some patients with type 2 diabetes. Nevertheless, the therapeutic value of the PPARγ nuclear receptor protein is limited due to the secondary effects of some PPARγ ligands. Becau...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572013005000002
更新日期:2013-03-01 00:00:00
abstract::The arylamine N-acetyltransferase 2 (NAT2) enzymes detoxify a wide range of naturally occurring xenobiotics including carcinogens and drugs. Point mutations in the NAT2 gene result in the variant alleles M1 (NAT2 *5A), M2 (NAT2*6A), M3 (NAT2*7) and M4 (NAT2 *14A) from the wild-type WT (NAT2 *4) allele. The current stu...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572012005000074
更新日期:2012-12-01 00:00:00
abstract::In order to understand the genetic ancestry and mitochondrial DNA (mtDNA) diversity of current Colombian horse breeds we sequenced a 364-bp fragment of the mitocondrial DNA D-loop in 116 animals belonging to five Spanish horse breeds and the Colombian Paso Fino and Colombian Creole cattle horse breeds. Among Colombian...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572012005000064
更新日期:2012-12-01 00:00:00
abstract::Several phylogeographic studies of seabirds have documented low genetic diversity that has been attributed to bottleneck events or individual capacity for dispersal. Few studies have been done in seabirds on the Brazilian coast and all have shown low genetic differentiation on a wide geographic scale. The Kelp Gull is...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572012005000065
更新日期:2012-12-01 00:00:00
abstract::Diacylglycerol O-acyltransferase 1 (DGAT1) is a microsomal enzyme that catalyzes the final step of triglyceride synthesis. The DGAT1 gene is a strong functional candidate for determining milk fat content in cattle. In this work, we used PCR-SSCP (polymerase chain reaction-single-strand conformation polymorphism) and D...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572012005000043
更新日期:2012-07-01 00:00:00
abstract::The purpose of this study was to investigate whether treatment with electroacupuncture (EA) inhibited mitochondria-dependent apoptosis in annulus fibrosis (AF) cells in a rat model of cervical intervertebral disc degradation induced by unbalanced dynamic and static forces. Forty Sprague-Dawley rats were used in this s...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572012005000046
更新日期:2012-07-01 00:00:00
abstract::Heat shock (HS) leads to the activation of molecular mechanisms, known as HS-response, that prevent damage and enhance survival under stress. Plants have a flexible and specialized network of Heat Shock Factors (HSFs), which are transcription factors that induce the expression of heat shock proteins. The present work ...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572012000200006
更新日期:2012-06-01 00:00:00
abstract::Sample preparation is a critical step in two-dimensional gel electrophoresis (2-DE) of plant tissues. Here we describe a phenol/SDS procedure that, although greatly simplified, produced well-resolved and reproducible 2-DE profiles of protein extracts from soybean [Glycine max (L.) Merril] roots. Extractions were made ...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572012000200016
更新日期:2012-06-01 00:00:00
abstract::A 10-year-old speechless, mentally deficient male, with low arylsulfatase A (ARSA) activity, and presumably, methachromatic leukodystrophy, underwent genetic evaluation. As the clinical picture was not compatible with this diagnosisan ARSA gene and chromosome analysis were performed, showing the presence of a pseudode...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572012000300007
更新日期:2012-04-01 00:00:00
abstract::Tabebuia sp. is native to tropical rain forests throughout Central and South America. Although the biological and pharmacological effects of bark extracts have been intensely studied, little is known on the extract obtained from the flower. Herein, the genotoxic potential of a flower extract from T. impetiginosa ("ipê...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572012005000030
更新日期:2012-04-01 00:00:00
abstract::Since around 1723, on the occasion of its initial colonization by Europeans, Rondonia has received successive waves of immigrants. This has been further swelled by individuals from northeastern Brazil, who began entering at the beginning of the twentieth century. The ethnic composition varies across the state accordin...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/s1415-47572012005000003
更新日期:2012-01-01 00:00:00
abstract::In the present study, water buffalo MHC (Bubu)-DRB cDNA was cloned and characterized. The 1022 base long-amplified cDNA product encompassed a single open reading frame of 801 bases that coded for 266 amino acids. The Bubu-DRB sequence showed maximum homology with the BoLA-DRB3*0101 allele of cattle. A total of seven a...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/s1415-47572012005000013
更新日期:2012-01-01 00:00:00
abstract::The antitumorigenic potential of two palladium(II) complexes, [Pd(ca(2)-o-phen)Cl(2)] - C1 and [Pd(dmba)(dppp)Cl] - C2, was evaluated, using MDA-MB-435 cells, a human breast adenocarcinoma cell-line that does not express the estrogen receptor α (ER-). Growth inhibition and induced alterations in cell-morphology were a...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/s1415-47572012005000016
更新日期:2012-01-01 00:00:00
abstract::Now a days, an important and interesting alternative in the control of tick-infestation in cattle is to select resistant animals, and identify the respective quantitative trait loci (QTLs) and DNA markers, for posterior use in breeding programs. The number of ticks/animal is characterized as a discrete-counting trait,...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572011005000049
更新日期:2011-10-01 00:00:00
abstract::A detailed study of putative recombination events and their evolution frequency in the whole genome of the currently known members of the family Tombusviridae, comprising 79 accessions retrieved from the international databases, was carried out by using the RECCO and RDP version 3.31β algorithms. The first program all...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572011005000046
更新日期:2011-10-01 00:00:00
abstract::The applicability of mitochondrial nad6 sequences to studies of DNA and population variability in Lepidoptera was tested in four species of economically important moths and one of wild butterflies. The genetic information so obtained was compared to that of cox1 sequences for two species of Lepidoptera. nad6 primers a...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572011000400028
更新日期:2011-10-01 00:00:00
abstract::Screening of mutations that cause β-thalassaemia in the Bangladeshi population led to the identification of a patient with a combination of two rare mutations, Hb Monroe and HBB: -92 C > G. The β-thalassaemia major male individual was transfusion-dependent and had an atypical β-globin gene cluster haplotype. Of the tw...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572011005000026
更新日期:2011-07-01 00:00:00